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KMID : 0861020130280030001
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Korea Journal of Herbology
2013 Volume.28 No. 3 p.1 ~ p.5
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Effects of Polygonati Rhizoma Extracts on the Collagenase Activity and Procollagen Synthesis in Hs68 Human Fibroblasts and Tyrosinase Activity
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:Park Dong-Su
:Shin Seon-Mi/:Leem Kang-Hyun
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Abstract
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Objectives： This study was designed to investigate the collagen metabolism and tyrosinase activity of
Polygonati Rhizoma extracts (PR). It's effects are to tonify spleen qi and augment the spleen yin. It enrichs the
yin and moisten the lung.
Methods : The effect of PR on type I procollagen production and collagenase (matrix metalloproteinase-1,
henceforth referred as MMP-1) activity in human normal fibroblasts Hs68 after ultraviolet B (UVB, 312 nm)
irradiation was measured by ELISA method. The tyrosinase activity after treatment of PR was measured.
Results : There were no cytotoxicity at concentrations of 10, 30, 100 μg/ml. The reduced type I procollagen
production was recovered by PR in UVB damaged Hs68 cells at a concentration of 100 μg/ml (16.2 ± 0.0
ng/ml) from control group (13.9 ± 0.5 ng/ml). However there was no statistical significance. PR reduced The
increased MMP-1 activity after UVB damage at concentrations of 10 μg/ml, 30 μg/ml, and 100 μg/ml in a dose
dependent manner (42.2 ± 20.5%, 44.8 ± 8.5%, and 22.0 ± 5.8%). PR 100 μg/ml treatment showed the
statistical significace (p < 0.05). PR significantly reduced the tyrosinase activity at a concentration of 10 mg/ml
(32.0 ± 12.8%, p < 0.05). However, the L-DOPA oxidation was not changed.
Conclusion : PR showed the anti-wrinkle effects and whitening effects in vitro. Although more researches are
needed to validate the efficacy, these results suggest that PR may have potential as an anti-aging ingredient
in cosmetic herb markets.
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KEYWORD
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Polygonati Rhizoma, type I procollagen, collagenase, tyrosinase
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